Following rinsing with PBS, cells were permeabilized with 0.3% Triton-X 100 (Sigma) for 15 min. relative release of histamine and tryptase. A: toluidine blue staining of peritoneal mast cells. (a) control group; (b) compound 48/80-treated cells; (c) Ang-1 100 ng/ml-treated cells; (d) Soluble form of Tie2 (sTie-2)-treated cells and (e) ON-013100 RGD-treated cells. B: Statistical … Continue reading Following rinsing with PBS, cells were permeabilized with 0